Coding

Part:BBa_K4877017

Designed by: Team iGEM Bielefeld-CeBiTec 2023   Group: iGEM23_Bielefeld-CeBiTec   (2023-10-12)


mNeonGreen flanked by additional SphI and SalI sites

mNeonGreen fluorescent reporter protein sequence with additional N-terminal SphI and C-terminal SalI restriction sites. An illegal PstI restriction site was silently mutated.

See Part:BBa_M50002 for an mNeonGreen sequence without SphI and SalI restriction sites.

The original sequence was taken from the published pDV06 plasmid by Gayet et al. [1], and modified to include the restriction sites.

[1] Gayet, R.V., Ilia, K., Razavi, S. et al. Autocatalytic base editing for RNA-responsive translational control. Nat Commun 14, 1339 (2023). https://doi.org/10.1038/s41467-023-36851-z

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None